Rozprawa doktorska Promotor: prof dr hab med. Janusz Marcinkiewicz



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9. Abstract

Taurine is a sulfonic β-amino acid of a significant regulatory role in human organism – it is necessary for proper development of central nervous system as well as for function of reproductive and immune system. Numerous observations indicate its particular importance for proper function of granulocytes. Studies performed over last 10 years have demonstrated that taurine haloamines (chloramine – TauCl – and bromamine – TauBr), synthesized in inflammatory reaction by activated neutrophils and eosinophils, show in vitro considerable immunoregulatory and antibacterial properties. Application of TauCl in topical treatment of infections (mostly in ophthalmology and laryngology) is presently studied in phase II clinical trials.

The aim of this work was to investigate and compare these properties of both compounds, which may give reasons for possible therapeutic application of TauBr in acute inflammatory conditions.

Stability of TauCl and TauBr in acellular environment, at various conditions of pH and temperature has been investigated. Cytotoxicity of both compounds has been studied and maximum non-cytotoxic concentrations determined, both for in vitro cell cultures and in vivo application at the site of acute inflammation. An attempt has been made to determine the mechanism of TauCl transport across the cellular membrane. Influence of TauCl and TauBr in vitro on production of cytokines and nitric oxide by inflammatory cells has been studied. Influence of both compounds on acute inflammatory reaction in vivo has been investigated.



Both studied taurine derivatives show significant effect in acute inflammatory reaction; TauBr as compared to TauCl is characterized by weaker anti-inflammatory properties and stronger antibacterial action. This observation as well as greater stability of TauCl indicates that TauCl is a more promising compound as related to clinical application.

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